请大家帮忙翻译一下这个段落
答案:1 悬赏:60 手机版
解决时间 2021-01-04 18:05
- 提问者网友：孤凫
- 2021-01-03 22:21
请大家帮忙翻译一下这个段落
最佳答案
- 五星知识达人网友：孤独入客枕
- 2021-01-03 23:22
Proteomic analysis 蛋白质组分析
Serum samples were thawed, added to a C16 hydrophobic interaction protein chip, and analysed on the Protein Biology System 2 SELDI-TOF mass spectrometer (Ciphergen Biosystems, Freemont, CA, USA).9 Mass resolution (defined as m/Δm) is routinely achieved below 400. Mass accuracy is assessed daily through the use of angiotensin peptide calibrations.
将血清试样解冻后，置于一个C16疏水性蛋白芯片，然后用美国加尼福尼亚费蒙市赛弗吉公司生产的2 SELDI-TOF蛋白质指纹质谱仪进行检测分析。低于400时的质量分辨率（定义为m/Δm）经常可达到9；质量准确性通过使用肽类血管紧张素校准每日进行测定。
We achieve a mass accuracy of 0?1% with this system. Peptides and proteins below the 20 000 mass/charge (M/Z) range were ionised with α-cyano-4-hydroxycinnamic acid as a matrix, which is most effective for the detection of proteins and peptides in this mass range. The chips were analysed manually under the following settings: laser intensity 240, detector sensitivity 10, mass focus 6000, position 50, molecular mass range 0–20 000 Da, and a 50-shot average per sample.
通过用本系统我们获得0.1%的质量准确性。低于20000质荷比（M/Z）范围的肽与蛋白质被α-cyano-4-羟基苯丙烯酸电离作为基质，这是最有效检测在这质量范围内的蛋白质与肽。
这些芯片以下列的设置被进行手工分析：激光强度240、检测器灵敏度10、质量焦点6000、位置50、分子质量范围0-20000 Da 以及对每个试样平均进行50次射击。
Data were collected without filters and were later used for analyses. Positives and controls were run concurrently, intermingled on the same chip and on multiple chips; the operators were unaware of which was which. None of the samples in the preliminary set were subsequently used in the masked validation set.
数据......余下全文>>
Serum samples were thawed, added to a C16 hydrophobic interaction protein chip, and analysed on the Protein Biology System 2 SELDI-TOF mass spectrometer (Ciphergen Biosystems, Freemont, CA, USA).9 Mass resolution (defined as m/Δm) is routinely achieved below 400. Mass accuracy is assessed daily through the use of angiotensin peptide calibrations.
将血清试样解冻后，置于一个C16疏水性蛋白芯片，然后用美国加尼福尼亚费蒙市赛弗吉公司生产的2 SELDI-TOF蛋白质指纹质谱仪进行检测分析。低于400时的质量分辨率（定义为m/Δm）经常可达到9；质量准确性通过使用肽类血管紧张素校准每日进行测定。
We achieve a mass accuracy of 0?1% with this system. Peptides and proteins below the 20 000 mass/charge (M/Z) range were ionised with α-cyano-4-hydroxycinnamic acid as a matrix, which is most effective for the detection of proteins and peptides in this mass range. The chips were analysed manually under the following settings: laser intensity 240, detector sensitivity 10, mass focus 6000, position 50, molecular mass range 0–20 000 Da, and a 50-shot average per sample.
通过用本系统我们获得0.1%的质量准确性。低于20000质荷比（M/Z）范围的肽与蛋白质被α-cyano-4-羟基苯丙烯酸电离作为基质，这是最有效检测在这质量范围内的蛋白质与肽。
这些芯片以下列的设置被进行手工分析：激光强度240、检测器灵敏度10、质量焦点6000、位置50、分子质量范围0-20000 Da 以及对每个试样平均进行50次射击。
Data were collected without filters and were later used for analyses. Positives and controls were run concurrently, intermingled on the same chip and on multiple chips; the operators were unaware of which was which. None of the samples in the preliminary set were subsequently used in the masked validation set.
数据......余下全文>>
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