【dmem培养基】DMEM培养基培养什么细胞可以培养F81嘛
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解决时间 2021-01-31 23:19
- 提问者网友：容嬷嬷拿针来
- 2021-01-31 18:45
【dmem培养基】DMEM培养基培养什么细胞可以培养F81嘛
最佳答案
- 五星知识达人网友：酒者煙囻
- 2021-01-31 19:11
【答案】 可以养很多细胞的,DMEM（意思就是双倍（double） 盐的 MEM）比MEM营养更丰富,DMEM又分为好多种的.
常用细胞所用培养基配方
CELL LINE CELL TYPE SPECIES TISSUE MEDIUM
293 fibroblast human embryonic kidney MEM,10% HI horse serum
3T6 fibroblast mouse embryo DMEM,10% FBS
A549 epithelial human lung carcinoma F-12K,10% FBS
A9 fibroblast mouse connective tissue DMEM,10% FBS
AtT-20 epithelial mouse pituitary tumor F-10,15% horse serum and 2.5% FBS
BALB/3T3 fibroblast mouse embryo DMEM,10% FBS
BHK-21 fibroblast hamster kidney GMEM,10% FBS or MEM,10% FBS and NEAA
BHL-100 epitheliall human breast McCoy'5A,10% FBS
BT fibroblast bovine turbinate cells MEM,10% FBS and NEAA
Caco-2 epithelial human colon adeno carcinoma MEM,20% FBS and NEAA
Chang epithelial human liver BME,10% calf serum
CHO-K1 epithelial hamster ovary F-12,10% FBS
Clone 9 epithelial rat liver F-12K,10% FBS
Clone M-3 epithelial mouse melanoma F-10,15% horse serum and 2.5% FBS
COS-1 fibroblast monkey kidney DMEM,10% FBS
COS-3 fibroblast monkey kidney DMEM,10% FBS
COS-7 fibroblast monkey kidney DMEM,10% FBS
CRFK epithelial cat kidney MEM,10% FBS and NEAA
CV-1 fibroblast monkey kidney MEM,10% FBS
D-17 epithelial dog osteosarcoma MEM,10% FBS and NEAA
Daudi lymphoblast human blood from a lymphoma patient RPMI-1640,10% FBS
GH1 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
GH3 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
H9 lymphoblast human T-cell lymphoma RPMI-1640,20% FBS
HaK epithelial hamster kidney BME,10% calf serum
HCT-15 epithelial human colorectal adenocarcinoma RPMI-1640,10% FBS
HeLa epithelial human cervix carcinoma MEM,10% FBS and NEAA (in suspension,S-MEM)
HEp-2 epithelial human larynx carcinoma MEM,10% FBS
HL-60 lymphoblast human promyeolocytic leukemia RPMI-1640,20% FBS
HT-1080 epithelial human fibrosarcoma MEM,10% HI FBS and NEAA
HT-29 epithelial human colon adenocarcinoma McCoy's 5A,10% FBS
HUVEC endothelial human umbilical cord F-12K,10% FBS and heparinat 100 ug ml
I-10 epithelial mouse testicular tumor F-10,15% horse serum and 2.5% FBS
IM-9 lymphoblast human marrow from myeloma patient RPMI-1640,10% FBS
JEG-2 epithelial human choriocarcinoma MEM,10% FBS
Jensen fibroblast rat sarcoma McCoy's 5A,5% FBS
Jurkat lyphoblast human lymphoma RPMI-1640,10% FBS
K-562 lymphoblast human myelogenous leukemia RPMI-1640,10% FBS
KB epithelial human oral carcinoma MEM,10% FBS and NEAA
KG-1 myeloblast human marrow from erythroleukemia patient IMDM,20% FBS
L2 epithelial rat lung F-12K,10%FBS
L6 rat skeletal muscle myoblasts DMEM,10% FBS
LLC-WRC 256 epithelial rat carcinoma Medium 199,5% horse serum
McCoy fibroblast mouse unknown MEM,10% FBS
MCF7 epithelial human breast adenocarcinoma MEM,10% FBS NEAA,10ug/ml insulin
WEHI-3b macrophage-like mouse myelomonocyte leukemia DMEM,10% FBS
WI-38 epithelial human embryonic lung BME,10% FBS
WISH epithelial human amnion BME,10% FBS
WS1 human embryonic skin MEM,10% FBS and NEAA
XC epithelial rat sarcoma MEM,10% FBS and NEAA
Y-1 epithelial mouse tumor of adrenal F-10,15% horse serum and 2.5% FBS
常用细胞所用培养基配方
CELL LINE CELL TYPE SPECIES TISSUE MEDIUM
293 fibroblast human embryonic kidney MEM,10% HI horse serum
3T6 fibroblast mouse embryo DMEM,10% FBS
A549 epithelial human lung carcinoma F-12K,10% FBS
A9 fibroblast mouse connective tissue DMEM,10% FBS
AtT-20 epithelial mouse pituitary tumor F-10,15% horse serum and 2.5% FBS
BALB/3T3 fibroblast mouse embryo DMEM,10% FBS
BHK-21 fibroblast hamster kidney GMEM,10% FBS or MEM,10% FBS and NEAA
BHL-100 epitheliall human breast McCoy'5A,10% FBS
BT fibroblast bovine turbinate cells MEM,10% FBS and NEAA
Caco-2 epithelial human colon adeno carcinoma MEM,20% FBS and NEAA
Chang epithelial human liver BME,10% calf serum
CHO-K1 epithelial hamster ovary F-12,10% FBS
Clone 9 epithelial rat liver F-12K,10% FBS
Clone M-3 epithelial mouse melanoma F-10,15% horse serum and 2.5% FBS
COS-1 fibroblast monkey kidney DMEM,10% FBS
COS-3 fibroblast monkey kidney DMEM,10% FBS
COS-7 fibroblast monkey kidney DMEM,10% FBS
CRFK epithelial cat kidney MEM,10% FBS and NEAA
CV-1 fibroblast monkey kidney MEM,10% FBS
D-17 epithelial dog osteosarcoma MEM,10% FBS and NEAA
Daudi lymphoblast human blood from a lymphoma patient RPMI-1640,10% FBS
GH1 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
GH3 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
H9 lymphoblast human T-cell lymphoma RPMI-1640,20% FBS
HaK epithelial hamster kidney BME,10% calf serum
HCT-15 epithelial human colorectal adenocarcinoma RPMI-1640,10% FBS
HeLa epithelial human cervix carcinoma MEM,10% FBS and NEAA (in suspension,S-MEM)
HEp-2 epithelial human larynx carcinoma MEM,10% FBS
HL-60 lymphoblast human promyeolocytic leukemia RPMI-1640,20% FBS
HT-1080 epithelial human fibrosarcoma MEM,10% HI FBS and NEAA
HT-29 epithelial human colon adenocarcinoma McCoy's 5A,10% FBS
HUVEC endothelial human umbilical cord F-12K,10% FBS and heparinat 100 ug ml
I-10 epithelial mouse testicular tumor F-10,15% horse serum and 2.5% FBS
IM-9 lymphoblast human marrow from myeloma patient RPMI-1640,10% FBS
JEG-2 epithelial human choriocarcinoma MEM,10% FBS
Jensen fibroblast rat sarcoma McCoy's 5A,5% FBS
Jurkat lyphoblast human lymphoma RPMI-1640,10% FBS
K-562 lymphoblast human myelogenous leukemia RPMI-1640,10% FBS
KB epithelial human oral carcinoma MEM,10% FBS and NEAA
KG-1 myeloblast human marrow from erythroleukemia patient IMDM,20% FBS
L2 epithelial rat lung F-12K,10%FBS
L6 rat skeletal muscle myoblasts DMEM,10% FBS
LLC-WRC 256 epithelial rat carcinoma Medium 199,5% horse serum
McCoy fibroblast mouse unknown MEM,10% FBS
MCF7 epithelial human breast adenocarcinoma MEM,10% FBS NEAA,10ug/ml insulin
WEHI-3b macrophage-like mouse myelomonocyte leukemia DMEM,10% FBS
WI-38 epithelial human embryonic lung BME,10% FBS
WISH epithelial human amnion BME,10% FBS
WS1 human embryonic skin MEM,10% FBS and NEAA
XC epithelial rat sarcoma MEM,10% FBS and NEAA
Y-1 epithelial mouse tumor of adrenal F-10,15% horse serum and 2.5% FBS
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- 1楼网友：第四晚心情
- 2021-01-31 20:09
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