0.1M，pH8.0的硼酸缓冲液怎么配制？
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解决时间 2021-03-30 03:24
- 提问者网友：呐年旧曙光
- 2021-03-29 15:44
0.1M，pH8.0的硼酸缓冲液怎么配制？
最佳答案
- 五星知识达人网友：夜风逐马
- 2021-03-29 17:00
直接用0.1M硼酸溶液滴加氢氧化钠到pH8.0
这样：
SODIUM BORATE BUFFER
50x Stock: 20 g NaOH
pH to 8.0 with approx 120 g H3BO3 (boric acid powder)
bring to 1 L with dH2O
NOTE: This stock will precipitate over time, requiring heat to re-dissolve crystallized salts before dilution.
1x Working Buffer: 20 ml of 50x stock
980 ml dH2O
NOTE: The pH of the working solution will be higher than that of the 50x stock
NOTE: Do not re-use 1x solution for more than a day or it will go fuzzy. Really.
Translated to concentrations:
50x Stock: 500 mM NaOH, pHed to 8.0 w/ H3BO3
1x Working buffer: 10 mM NaOH, pHed to 8.5 w/ H3BO3
这样：
SODIUM BORATE BUFFER
50x Stock: 20 g NaOH
pH to 8.0 with approx 120 g H3BO3 (boric acid powder)
bring to 1 L with dH2O
NOTE: This stock will precipitate over time, requiring heat to re-dissolve crystallized salts before dilution.
1x Working Buffer: 20 ml of 50x stock
980 ml dH2O
NOTE: The pH of the working solution will be higher than that of the 50x stock
NOTE: Do not re-use 1x solution for more than a day or it will go fuzzy. Really.
Translated to concentrations:
50x Stock: 500 mM NaOH, pHed to 8.0 w/ H3BO3
1x Working buffer: 10 mM NaOH, pHed to 8.5 w/ H3BO3
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