【onespo】...oncoldstoragehastwokindsofdifferentgraymoldsymptoms:onespo...
答案:2 悬赏:0 手机版
解决时间 2021-03-05 02:10
- 提问者网友：献世佛
- 2021-03-04 14:06
【onespo】...oncoldstoragehastwokindsofdifferentgraymoldsymptoms:onespo...
最佳答案
- 五星知识达人网友：愁杀梦里人
- 2021-03-04 14:41
【答案】 1,Post-harvested grape berries on cold storage manifest two kinds of different gray mold symptoms:the first sporulate on grape berries rapidly and develop a large amount of spores,cause the grape to decay rapidly,the second develop no spores on grape berries,only mycelium.
5,Using species-specific primers to amplify B.cinerea species-specific fragments showed that only EXFG Ⅲ displays this fragment with differences in sequence among strains,and confirms the morphological classification result that EXFG Ⅲ is a typical B.cinerea.Further amplification of the 1171bp fragment of B.cinerea Bc-hch gene show that EXFG Ⅱ and EXFG Ⅲ have this fragment,while HhaI digestion shows no difference in sequence.These results show that EXFG Ⅱ and EXFG Ⅲ may be classifeid as two different subspecies of B.cinerea.EXFG Ⅰ has no B.cinerea specific sequence,and may be just another species of the genus Botrytis.
6,The results of the analysis of the differences in rDNA-ITS sequence of 3 groups,show that the rDNA-ITS sequence of 3 groups differ only in one or two bases,and could not identify the strains of 3 groups base on rDNA-ITS sequence analysis.ISSR(Inter-Simple Sequence Repeat) cluster analysis show the genetic differences between strains from 3 groups.The results show that EXFG Ⅰhad a genetic distance of 0.89 compared with two other groups.Genetic distance between EXFG Ⅱand B.cinerea group EXFG Ⅲ was 0.74.The results show that strains of 3 groups have large genetic differences,and confirmed the classification of groups on the basis of morphological criteria.
7,In order to establish a method for rapid detection of the highly pathogenic group EXFG Ⅱ,we have developed a pair of specific molecular markers GBOT-20±by the use of the SCAR technology.GBOT-20±can be used for the specific detection and quantitative analysis of grapes gray mold EXFG Ⅱ on grape plant or post-harvested grape berries.
To sum up,gray mold pathogens on table grape can be divided into 3 groups.EXFG Ⅱ is different from the above mentioned gray mold pathogen (B.cinerea) in its morphology,sporulation time and symptoms,as well as genetic characteristics.EXFG Ⅱ have high pathogenicity and infect a wide range of hosts and sporulate rapidly on grape berries.It is a very dangerous new group of plant infectants.EXFG Ⅰ is possibly another species of the genus Botrytis.EXFG Ⅲ is a typical gray mold pathogen (Botrytis cinerea Pers.:Fr.) as previously mentioned.This study provides useful reference for the classification of species or subspecies related to B.cinerea,and provides an important basis for adequate control measures.
5,Using species-specific primers to amplify B.cinerea species-specific fragments showed that only EXFG Ⅲ displays this fragment with differences in sequence among strains,and confirms the morphological classification result that EXFG Ⅲ is a typical B.cinerea.Further amplification of the 1171bp fragment of B.cinerea Bc-hch gene show that EXFG Ⅱ and EXFG Ⅲ have this fragment,while HhaI digestion shows no difference in sequence.These results show that EXFG Ⅱ and EXFG Ⅲ may be classifeid as two different subspecies of B.cinerea.EXFG Ⅰ has no B.cinerea specific sequence,and may be just another species of the genus Botrytis.
6,The results of the analysis of the differences in rDNA-ITS sequence of 3 groups,show that the rDNA-ITS sequence of 3 groups differ only in one or two bases,and could not identify the strains of 3 groups base on rDNA-ITS sequence analysis.ISSR(Inter-Simple Sequence Repeat) cluster analysis show the genetic differences between strains from 3 groups.The results show that EXFG Ⅰhad a genetic distance of 0.89 compared with two other groups.Genetic distance between EXFG Ⅱand B.cinerea group EXFG Ⅲ was 0.74.The results show that strains of 3 groups have large genetic differences,and confirmed the classification of groups on the basis of morphological criteria.
7,In order to establish a method for rapid detection of the highly pathogenic group EXFG Ⅱ,we have developed a pair of specific molecular markers GBOT-20±by the use of the SCAR technology.GBOT-20±can be used for the specific detection and quantitative analysis of grapes gray mold EXFG Ⅱ on grape plant or post-harvested grape berries.
To sum up,gray mold pathogens on table grape can be divided into 3 groups.EXFG Ⅱ is different from the above mentioned gray mold pathogen (B.cinerea) in its morphology,sporulation time and symptoms,as well as genetic characteristics.EXFG Ⅱ have high pathogenicity and infect a wide range of hosts and sporulate rapidly on grape berries.It is a very dangerous new group of plant infectants.EXFG Ⅰ is possibly another species of the genus Botrytis.EXFG Ⅲ is a typical gray mold pathogen (Botrytis cinerea Pers.:Fr.) as previously mentioned.This study provides useful reference for the classification of species or subspecies related to B.cinerea,and provides an important basis for adequate control measures.
全部回答
- 1楼网友：十年萤火照君眠
- 2021-03-04 16:18
这下我知道了
我要举报
如以上回答内容为低俗、色情、不良、暴力、侵权、涉及违法等信息，可以点下面链接进行举报！
点此我要举报以上问答信息
大家都在看
推荐资讯