动物组织DNA的提取及核酸浓度的测定
为什么要测310nm处的吸光值?细胞裂解液和沉淀液中的EDTA 有什么作用?氯化钠和醋酸钠有什么作用?
动物组织DNA的提取及核酸浓度的测定
答案:1 悬赏:30 手机版
解决时间 2021-08-14 08:09
- 提问者网友：像風在裏
- 2021-08-13 10:44
最佳答案
- 五星知识达人网友：一袍清酒付
- 2021-08-13 11:15
DNA concentration is measured by OD260,protein usually is OD280.Some proteins have peak absorbency around 310 nm.Therefore it can be used for judging protein contamination.
EDTA is able to chelate Mg2+ (Magnesium) which is required for most DNase.After Mg2+ is chelated,DNase will not work,this help to preserve DNA..
Sodium acetate and sodium chloride can help to precipitate DNA.
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